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KMID : 0861020190340030055
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Korea Journal of Herbology
2019 Volume.34 No. 3 p.55 ~ p.61
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Inhibitory effect of Cinnamomi Cortex extract on motility of prostate cancer cellsthrough reducing YAP activity
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Jung Hyo-Won
Kim Ok-Hyeon
Wang Tsu-Yu
Kim Seong-Eun
Park Yong-Kee
Lee Hyun-Jung
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Abstract
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Objectives : Recently, natural bioactive components catch a major attention for their potent anticarcinogenic activity. In this study, the inhibitory effect of Cinnamomi Cortex (CC) was examined in PC3 prostate cancer cells.
Methods : The toxicity of CC extract was evaluated with cell viability and cell morphology. The activity of Yes associated protein (YAP) was tested with qRT-PCR for the target gene expression such as CTGF and AMOTL2. Western blotting was performed for the evaluation of phospho-YAP level. For cell motility analysis, cellular motility was imaged by live imaging system for 6 hr. Successive images were used for the generation of movie file. Using this movie file, cellular migration was manually tracked and analyzed using time-lapse microscope and Fiji software.
Results : Cytotoxicity of CC extract was not detected at 500 ㎍/㎖ or below concentration. Although 500 ㎍/㎖ of CC extract reduced CTGF and AMOTL2 gene expression as YAP target genes, it was not statistically significant (CTGF expression P=0.0605, AMOTL2 expression P=0.4478). However, phosphorylated YAP was highly enhanced by CC extract treatment, when normalized with total YAP protein expression, suggesting YAP activation was inhibited. Finally prostate cancer cell motility was markedly reduced by 500 ㎍/㎖ of CC extract.
Conclusions : CC extract suppresses cancer cell motility and migration ability through inhibiting YAP activation without prostate cancer cell death, suggesting that this herb might be effective therapeutic drug for prostate cancer metastasis.
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KEYWORD
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Cinnamomum cassia, Cinnamomi Cortex, Prostate cancer cell, YAP, motility
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